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Human GLP-1 PharmaGenie ELISA Kit (SBRS0027)

SKU:
SBRS0027
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Competitive
€649
Frequently bought together:

Description

system_update_altDatasheet

Human GLP-1 PharmaGenie ELISA - Kit Information

Glucagon-like peptide-1 (GLP-1) is a 31 amino acid peptide hormone derived from selective cleavage of the proglucagon gene. It is mainly produced from enteroendocrine L-cells in GI tract. The other cleavage products derived from proglucagon genes are glucagon, GLP-2 and other small fragment peptides including Glicentin, Oxyntomodulin and two intervening peptides (IP-1 and IP-2). Except for glucagon cleaved in alpha cells of the pancreas, all other cleaved peptides occurred in enteroendocrine L cells of intestine. GLP-1 has shown important roles in regulating glucose metabolic functions in humans. There are studies showing that GLP-1 is a potent anti-hyperglycemic hormone inducing glucose-dependent stimulation of insulin secretion while suppressing glucagon secretion. This dual control of insulin and glucagon has the benefit that the plasma glucose concentration is kept in the normal fasting range to avoid hypoglycemia caused by overstimulation of insulin. In addition, GLP-1 was reported to restore the glucose sensitivity of pancreatic beta cells, probably via the upregualtion of GLUT2 and glucokinase. GLP-1 was reported to inhibit pancreatic beta-cell apoptosis and stimulate the proliferation and differentiation of insulin-secreting beta-cells. GLP-1 has shown potential clinical application as a biomarker and treatment option for Diabetes Mellitus, which is based on the following physiological functions mediated by GLP-1. Regulating insulin secretion by increasing insulin secretion from the pancreas in a glucose-dependent manner and increasing insulin-sensitivity in both alpha and beta cells Regulating glucagon secretion by decreasing glucagon secretion from the pancreas by engagement of a specific G protein-coupled receptor. Regulating food intake by inhibiting acid secretion and gastric emptying in the stomach and decreasing food intake by increasing satiety in brain.

Human GLP-1 PharmaGenie ELISA - Kit Data

Product SKU:SBRS0027
Size:96T
Application:Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
Uniprot:P01275
Gene ID:2641
Gene Names:GCG
Synonyms:Glucagon [Cleaved into: Glicentin Glicentin-related polypeptide (GRPP) Oxyntomodulin (OXM) (OXY) Glucagon Glucagon-like peptide 1 (GLP-1) (Incretin hormone) Glucagon-like peptide 1(7-37) (GLP-1(7-37)) Glucagon-like peptide 1(7-36) (GLP-1(7-36)) Glucagon-like peptide 2 (GLP-2)]
Target Species:Human, Mouse, Rat
Compatible Sample Types:Cell Culture Supernatants, Serum
Design Principle:Competition-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:0.1-1,000 pg/ml
Sensitivity:1.17 pg/ml
Recommended Dilution:Human: 2X, Mouse: 2X, Rat: 2X
Storage/Stability:Standard, Biotinylated GLP-1 peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Standard Peptide
4.Assay Diluent(s)
5.Biotinylated Peptide
6.HRP-Streptavidin
7.TMB One-Step Substrate
8.Stop Solution
9.Assay Diagram
10.Positive Control Sample
11.Capture Antibody
12.Technical Manual

Other materials and equipment required:

The Assay Genie Human GLP-1 ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Kit Protocol: