The Glucagon-Like Peptide-1 (GLP-1) ELISA Kit is specially designed for the accurate quantitative measurement of GLP-1 levels in human samples. GLP-1, a critical gut hormone, plays a key role in regulating glucose homeostasis and insulin release, making it a vital component of blood sugar control and metabolism. Monitoring GLP-1 levels is essential for understanding metabolic processes, particularly in conditions such as diabetes and metabolic disorders. This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for the quantification of GLP-1 in various biological samples. Manufactured under stringent quality control standards, the GLP-1 ELISA Kit provides reliable performance and user-friendly protocols, making it suitable for both research and clinical applications. Trust in the GLP-1 ELISA Kit from Assay Genie for accurate and dependable quantification of this essential biomarker in your scientific investigations.
Product Name:
Human GLP-1 (Glucagon Like Peptide 1) ELISA Kit
SKU:
AEES00061
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 pg/mL
Detection range:
1.56-100 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.