The Rat Glucagon Like Peptide 1 (GLP-1) ELISA Kit is a specialized assay meticulously crafted for the quantitative detection of GLP-1 levels in rat biological samples. GLP-1, a crucial hormone primarily produced in the intestines, plays a pivotal role in glucose homeostasis, insulin release, and appetite regulation. This ELISA kit provides researchers with a versatile tool to explore the dynamic functions of GLP-1 in metabolic processes and its possible implications in various physiological functions. Accurate measurement of GLP-1 levels is essential for unraveling its intricate involvement in glucose metabolism, insulin secretion, and energy balance. With the Rat GLP-1 ELISA Kit, researchers can delve deeper into the intricate mechanisms governed by GLP-1, shedding light on its therapeutic potential in metabolic disorders and diabetes research. Engineered with high sensitivity and specificity, this ELISA kit ensures the generation of robust and reproducible results, empowering researchers to uncover novel insights into the regulatory roles of GLP-1 in metabolic pathways. Trust in the accuracy and reliability of the Rat GLP-1 ELISA Kit to propel your research forward and enhance discoveries in the domain of metabolic physiology and endocrine regulation.
Product Name:
Rat GLP-1 (Glucagon Like Peptide 1) ELISA Kit
SKU:
AEES00523
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 pg/mL
Detection range:
1.56-100 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
