The Human GLP-1 (Glucagon-Like Peptide-1) CLIA Kit is a highly reliable tool for the precise measurement of GLP-1 levels in human samples, including serum, plasma, and cell culture supernatants. This innovative kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.GLP-1 is a key regulatory peptide involved in glucose homeostasis, satiety, and insulin secretion. Dysregulation of GLP-1 signaling is associated with metabolic disorders such as type 2 diabetes, making it a vital target for therapeutic interventions.
By quantifying GLP-1 levels in biological samples, researchers can gain valuable insights into disease mechanisms and potential treatment strategies.Whether investigating the role of GLP-1 in metabolic diseases or exploring its therapeutic potential, the Human GLP-1 CLIA Kit provides researchers with a powerful tool for advancing their scientific endeavors. Trust in the precision and reliability of this kit to support your research goals with accurate and reproducible results.
Product Name:
Human GLP-1 (Glucagon Like Peptide 1) CLIA Kit
SKU:
HUES00131
Target:
Human GLP-1 (Glucagon Like Peptide 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GLP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GLP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GLP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GLP-1. You can calculate the concentration of Human GLP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-117
94-110
89-101
Average (%)
108
101
95
1:4
Range (%)
88-102
100-115
99-112
Average (%)
94
107
106
1:8
Range (%)
98-114
99-115
99-113
Average (%)
105
106
105
1:16
Range (%)
94-112
99-116
94-106
Average (%)
102
107
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
99
EDTA plasma (n=5)
95-110
102
Cell culture media (n=5)
94-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
266.48
463.63
2096.13
244.73
430.59
1991.37
Standard deviation
31.66
48.03
203.53
21.07
38.67
163.89
C V (%)
11.88
10.36
9.71
8.61
8.98
8.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GLP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GLP-1 in samples. No significant cross-reactivity or interference between Human GLP-1 and analogues was observed.
