Human Glutamate Decarboxylase 1, Brain (GAD1) ELISA Kit
The Human Glutamate Decarboxylase 1 (Brain GAD1) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of GAD1 levels in human serum, plasma, and cell culture supernatants. This kit is designed to provide reliable and reproducible results, making it ideal for a wide range of research applications.Glutamate decarboxylase 1 (GAD1) is an important enzyme involved in the synthesis of the neurotransmitter gamma-aminobutyric acid (GABA) in the brain. GAD1 plays a crucial role in the regulation of neuronal activity and has been implicated in disorders such as epilepsy, schizophrenia, and autoimmune diseases affecting the central nervous system.
As such, the Human Glutamate Decarboxylase 1 (Brain GAD1) ELISA Kit is a valuable tool for researchers studying these conditions and investigating potential therapeutic interventions.Overall, the Human Glutamate Decarboxylase 1 (Brain GAD1) ELISA Kit offers researchers a reliable and convenient method for quantifying GAD1 levels in human biological samples, providing valuable insights into the role of this enzyme in neurological and psychiatric disorders.
Product Name:
Human Glutamate Decarboxylase 1, Brain (GAD1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
