Human Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2) ELISA Kit
The Human Glutamate Receptor Ionotropic AMPA 2 (GRIA2) ELISA Kit is specifically designed for the precise detection of GRIA2 levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and consistent results, making it an essential tool for researchers in various fields.GRIA2 is a key component of the ionotropic glutamate receptor, playing a crucial role in neuronal communication and synaptic plasticity. Dysregulation of GRIA2 has been implicated in various neurological disorders, including Alzheimer's disease, epilepsy, and schizophrenia.
Therefore, the measurement of GRIA2 levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of novel therapeutic strategies.Overall, the Human Glutamate Receptor Ionotropic AMPA 2 (GRIA2) ELISA Kit offers a reliable and efficient solution for studying GRIA2 biology and its implications in neurological disorders, paving the way for groundbreaking discoveries in the field of neuroscience.
Product Name:
Human Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
