Human Golgin A8 Family, Member A (GOLGA8A) ELISA Kit
The Human Golgin A8 Family Member A (Golga8a) ELISA Kit is specifically designed for the quantitative detection of Golga8a levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, ensuring dependable and consistent results for various research purposes.Golga8a is a key protein belonging to the Golgin A8 family, known for its role in regulating protein transport within the Golgi apparatus. Dysregulation of Golga8a has been linked to several human diseases, including cancer and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With the Human Golga8a ELISA Kit, researchers can confidently measure Golga8a levels in biological samples, advancing their understanding of Golga8a's function and its implications in disease pathology. This kit is a valuable tool for investigating Golga8a-related research questions and developing targeted treatment strategies.
Product Name:
Human Golgin A8 Family, Member A (GOLGA8A) ELISA Kit
Product Code:
HUDL01222
Size:
96 Assays
Target:
GOLGA8A
Synonyms:
GM88, GOLGIN-67, Golgi Autoantigen, Golgin Subfamily A, 8A, 88 kDa Golgi matrix protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
84-102
93
EDTA plasma (n=5)
91-101
96
Heparin plasma (n=5)
92-98
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
