Description
Human GPC3 Recombinant Protein (N-His)
Human GPC3 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser359-Asn554, with an N-terminal His tag. Predicted molecular weight: 24.46 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Glypican-3 (GPC3/Glypican 3) is a ~65 kDa protein. Cell surface proteoglycan. For research use only.
Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway.
| Product Name: | Human GPC3 Recombinant Protein (N-His) |
| SKU: | GXRP01747 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ser359-Asn554 |
| Calculated MW: | 24.46 kDa |
| UniProt: | P51654 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
