Description
Human GRK5 Recombinant Protein (N-His)
Human GRK5 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Thr182-Asn450, with an N-terminal His tag. Predicted molecular weight: 33.16 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. G protein-coupled receptor kinase 5 is a ~67 kDa protein. Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). For research use only.
Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors.
| Product Name: | Human GRK5 Recombinant Protein (N-His) |
| SKU: | GXRP10975 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | EC:2.7.11.16, G protein-coupled receptor kinase 5, G protein-coupled receptor kinase GRK5, GPRK5, GRK5 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Thr182-Asn450 |
| Calculated MW: | 33.16 kDa |
| UniProt: | P34947 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
