Human Growth Differentiation Factor 2 (GDF2) ELISA Kit
The Human Growth Differentiation Factor 2 (GDF2) ELISA Kit is a powerful tool for the measurement of GDF2 levels in human samples, including serum, plasma, and cell culture supernatants. This advanced kit offers high precision and accuracy, ensuring dependable and reproducible results for a variety of research applications.GDF2, also known as Bone Morphogenetic Protein 9 (BMP-9), is a key regulator of growth and differentiation in various cell types. It plays a crucial role in multiple biological processes, including bone formation, cell differentiation, and tissue regeneration.
Dysregulation of GDF2 has been linked to numerous diseases and conditions, highlighting its importance as a potential biomarker for disease diagnosis and therapy development.With its cutting-edge technology and reliable performance, the Human GDF2 ELISA Kit is an indispensable tool for researchers studying growth factors, differentiation pathways, and therapeutic interventions. Get precise and insightful results with this top-notch kit from AssayGenie.
Product Name:
Human Growth Differentiation Factor 2 (GDF2) ELISA Kit
Product Code:
HUDL01168
Size:
96 Assays
Target:
GDF2
Synonyms:
BMP9, Bone Morphogenetic Protein 9
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
