Human HAMA-IgE (Human Anti-Mouse Ig Antibody IgE Class) ELISA Kit
The Human HAMA-IgE (Human Anti-Mouse Ig Antibody IgE Class) ELISA Kit from AssayGenie is a cutting-edge tool for precise measurement of HAMA-IgE levels in human serum, plasma, cell culture supernatant, and other biological samples. This assay delivers exceptional sensitivity and specificity, ensuring accurate quantification of target analytes across diverse sample matrices. Human anti-mouse antibodies (HAMA) of the IgE class represent a clinically significant humoral immune response, typically generated when patients are exposed to murine monoclonal antibodies or mouse-derived therapeutic agents. HAMA-IgE specifically mediates type I hypersensitivity reactions and is implicated in immediate allergic responses, anaphylaxis, and reduced efficacy of mouse-based immunotherapies in treated patients.
Equipped with this ELISA kit, researchers can delve deep into the intricate mechanisms governed by HAMA-IgE and gain valuable insights into its involvement in different biological pathways. Unleash the potential of your human research with the AssayGenie Human HAMA-IgE (Human Anti-Mouse Ig Antibody IgE Class) ELISA Kit—empowering innovation and discovery in the realm of molecular biology.
Product Name:
Human HAMA-IgE (Human Anti-Mouse Ig Antibody IgE Class) ELISA Kit
SKU:
AEFI04267
Reactivity:
Human
Detection Method:
Capture
Sensitivity:
0.938ng/ml
Range:
1.563-100ng/ml
Sample Type:
Serum, Plasma, Cell Culture Supernatant, And Other Biological Samples.
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum(n=5)
89-100%
92-100%
92-99%
EDTA Plasma(n=5)
90-105%
89-99%
92-100%
Heparin Plasma(n=5)
86-103%
90-95%
90-100%
Recovery:
Matrix
Recovery Range (%)
Average (%)
Serum(n=5)
96-103
100
EDTA Plasma(n=5)
94-97
96
Heparin Plasma(n=5)
93-104
98
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate to room temperature for at least 30 minutes. Designate standard, sample and control wells on the pre-coated plate.
2
Standard, Sample & Control Loading: Aliquot 100 µl of standard solutions, samples and Sample Dilution Buffer into the appropriate wells.
3
First Incubation & Wash: Seal the plate and incubate at 37°C for 90 minutes. Wash 2 times with Wash Buffer.
4
HRP-Streptavidin (SABC) & Wash: Add SABC working solution and incubate at 37°C for 30 minutes. Wash 5 times, allowing the buffer to stay in the wells for 1-2 minutes per wash.
5
Color Development: Add TMB substrate and incubate at 37°C in the dark for 10-20 minutes.
6
Stop Reaction & Reading: Add 50 µl Stop Solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow whole blood to coagulate at room temperature (2 h) or 2-8°C overnight. Centrifuge at 1000 × g for 20 min and collect the supernatant. Store or use immediately.
Plasma
Collect in anticoagulant tubes (EDTA, citrate, or heparin), mix gently, and centrifuge within 30 min at 1000 × g, 2-8°C for 15 min. Store or assay as needed.
Tissue Homogenate
Homogenize tissue in 50 mM Tris + 0.9% NaCl + 0.1% SDS (pH 7.3) with protease inhibitors (e.g. 1 mM PMSF). Avoid NP-40, Triton X-100 and DTT. Disrupt by ultrasonic homogenization or freeze-thaw cycles, centrifuge at 5000 × g for 5 min and collect the supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes at 2-8°C and collect the clarified cell culture supernatant.
Cell Lysate
Lyse cells in lysis buffer with protease inhibitor (e.g. 1 mM PMSF) on ice for 30-60 minutes, or by ultrasonic treatment. Centrifuge at 10,000 rpm for 10 min at 2-8°C and collect the supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Stored for 1 month at 2-8°C; Stored for 12month at -20°C
Lyophilized Standard
1 vial
2 vial
Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
