Human Heat Shock Transcription Factor 1 (HSF1) ELISA Kit
The Human Heat Shock Transcription Factor 1 (HSF1) ELISA Kit is a powerful tool for accurately measuring levels of HSF1 in human biological samples such as serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.HSF1 is a key regulator of the heat shock response and is involved in the cellular stress response. Dysregulation of HSF1 has been linked to various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
Thus, measuring HSF1 levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of potential therapeutic interventions.The Human HSF1 ELISA Kit from Assay Genie offers researchers a reliable and efficient solution for studying the role of HSF1 in health and disease. With its high-quality components and user-friendly protocol, this kit is a valuable asset for any laboratory conducting research on the molecular mechanisms underlying cellular stress response.
Product Name:
Human Heat Shock Transcription Factor 1 (HSF1) ELISA Kit
Product Code:
HUDL01376
Size:
96 Assays
Target:
HSF1
Synonyms:
HSTF1, Heat shock factor protein 1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
