Human Heterogeneous Nuclear Ribonucleoprotein U (HNRPU) ELISA Kit
The Human Heterogeneous Nuclear Ribonucleoprotein U (HNRPU) ELISA Kit is a reliable and accurate tool for the detection of HNRPU levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise results for research applications. HNRPU is a key protein involved in various cellular processes, including RNA splicing, transcriptional regulation, and DNA repair. Dysregulation of HNRPU expression has been implicated in a variety of diseases, such as cancer, autoimmune disorders, and neurological conditions.
Therefore, the Human HNRPU ELISA Kit is an essential tool for investigating the role of HNRPU in disease pathogenesis and developing potential therapeutic interventions.Overall, the Human HNRPU ELISA Kit is a valuable resource for researchers seeking to study the function of HNRPU in health and disease, providing accurate and reliable results for a diverse range of scientific investigations.
Product Name:
Human Heterogeneous Nuclear Ribonucleoprotein U (HNRPU) ELISA Kit
Product Code:
HUDL01350
Size:
96 Assays
Target:
HNRPU
Synonyms:
hnRNP-U, HNRNPU, SAF-A, U21.1, Scaffold Attachment Factor A
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
