Human High Mobility Group Nucleosome Binding Domain Protein 1 (HMGN1) ELISA Kit
The Human High Mobility Group Nucleosome-Binding Domain Protein 1 (HMGN1) ELISA Kit is a powerful tool for scientists studying the role of HMGN1 in various biological processes. This kit is specifically designed for the accurate measurement of HMGN1 levels in human serum, plasma, and cell culture supernatants.HMGN1 is a protein known for its involvement in chromatin structure regulation and gene expression. It plays a critical role in controlling DNA accessibility and is implicated in various disease processes, including cancer and inflammatory disorders.
With high sensitivity and specificity, this ELISA kit provides researchers with reliable and reproducible results, enabling in-depth investigations into the role of HMGN1 in health and disease. Whether studying the molecular mechanisms of disease or developing potential therapeutic interventions, the Human HMGN1 ELISA Kit offers a valuable tool for advancing scientific research.
Product Name:
Human High Mobility Group Nucleosome Binding Domain Protein 1 (HMGN1) ELISA Kit
Product Code:
HUDL01345
Size:
96 Assays
Target:
HMGN1
Synonyms:
IPO38, HMG14, Non-histone chromosomal protein HMG-14
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
