Human HMGB-1 (High mobility group protein B1) ELISA Kit
The Human HMGB-1 (High Mobility Group Protein B1) ELISA Kit is a highly specific and sensitive assay designed for the accurate measurement of HMGB-1 levels in human serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it a valuable tool for a variety of research applications.HMGB-1 is a critical protein involved in various inflammatory and immune responses, playing a key role in conditions such as sepsis, arthritis, and cancer. By accurately measuring HMGB-1 levels, researchers can gain insights into disease progression, treatment efficacy, and potential therapeutic strategies.With its high sensitivity and specificity, the Human HMGB-1 ELISA Kit is an essential tool for studying the role of HMGB-1 in health and disease, offering researchers a reliable and efficient method for quantifying HMGB-1 levels in biological samples.
Product Name:
Human HMGB-1 (High mobility group protein B1) ELISA Kit
SKU:
HUES02568
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
