Rat HMGB-1 (High Mobility Group Protein B1) ELISA Kit
The Rat HMGB-1 (High Mobility Group Protein B1) ELISA Kit is a specialized assay designed for the precise quantitative detection of HMGB-1 levels in diverse biological samples. High Mobility Group Protein B1 is a vital nuclear protein that plays a multifaceted role as a DNA chaperone, modulator of transcriptional activity, and mediator of inflammation and immunity. It is often released into extracellular spaces during cellular stress, infection, and inflammatory processes, where it acts as a damage-associated molecular pattern (DAMP) to regulate immune responses. Accurate measurement of HMGB-1 levels is crucial for unraveling its involvement in diverse physiological and pathological conditions, ranging from inflammation to cancer. The Rat HMGB-1 ELISA Kit provides exceptional sensitivity and specificity, enabling researchers to obtain reliable and reproducible quantitative data. Manufactured under stringent quality control standards, this kit ensures robust performance and ease of use, making it an indispensable tool for investigating the intricate roles of HMGB-1 in health and disease.
Product Name:
Rat HMGB-1 (High Mobility Group Protein B1) ELISA Kit
SKU:
AEES00435
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
