Human IFI30 Recombinant Protein (N-His) (GXRP00931)

Human IFI30 Recombinant Protein (N-His)

Human IFI30 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Lys250, with an N-terminal His tag. Predicted molecular weight: 30.13 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Gamma-interferon-inducible lysosomal thiol reductase (IFI30) is a ~27 kDa protein. Lysosomal thiol reductase that catalyzes protein disulfide bonds reduction. For research use only.

Plays an important role in antigen processing and presentation, of namely both major histocompatibility complex (MHC) class I- and class II-restricted antigen by reducing the disulfide bonds of endocytosed proteins and facilitating their unfolding and optimal degradation. Shares with thioredoxin a reduction mechanism in which the N-terminal cysteine thiol group in the CXXC active site motif initiates a nucleophilic attack on the substrate disulfide bond,resulting in the formation of a mixed disulfide-linked enzyme-substrate intermediate (Probable). Subsequent intramolecular attack by the second cysteine thiol group enables the release of the reduced substrate and oxidized enzyme (Probable). Lysosomal cysteine is a physiological reducing agent that is capable of reducing IFI30, so that it can catalyzes the next reaction.