The Human IFN-Beta (Interferon-Beta) CLIA Kit is specifically designed for the quantitative measurement of IFN-Beta levels in human samples such as serum, plasma, and cell culture supernatants. This CLIA kit offers high sensitivity and specificity, ensuring accurate and reliable results for researchers working in the fields of immunology, virology, and inflammatory diseases.Interferon-Beta is a key cytokine involved in the immune response, particularly in viral infections and autoimmune diseases.
Its dysregulation has been linked to conditions such as multiple sclerosis, making it a valuable biomarker for studying disease progression and treatment outcomes.With this CLIA kit, researchers can easily and efficiently analyze IFN-Beta levels in human samples, providing valuable insights into immune responses and disease pathways. Trust in the Human IFN-Beta CLIA Kit for precise and dependable results in your research endeavors.
Product Name:
Human IFN- beta (Interferon Beta) CLIA Kit
SKU:
HUES00082
Target:
Human IFN- beta (Interferon Beta)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-β. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFN-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFN-β, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IFN-β. You can calculate the concentration of Human IFN-β in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
91-105
87-102
Average (%)
103
97
94
1:4
Range (%)
85-98
98-113
99-116
Average (%)
90
104
106
1:8
Range (%)
91-102
92-107
96-108
Average (%)
97
99
102
1:16
Range (%)
100-116
87-101
102-115
Average (%)
108
94
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
107
EDTA plasma (n=5)
85-97
91
Cell culture media (n=5)
93-109
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.59
78.18
179.87
22.38
73.56
197.71
Standard deviation
2.74
8.04
18.78
2.43
8.43
15.12
C V (%)
11.14
10.28
10.44
10.86
11.46
7.65
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IFN-β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IFN-β in samples. No significant cross-reactivity or interference between Human IFN-β and analogues was observed.
