The Human IFN-Gamma (Interferon-Gamma) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of IFN-Gamma levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it an essential tool for researchers studying immune responses and inflammatory diseases.IFN-Gamma is a key cytokine involved in the immune response, playing a crucial role in the activation of macrophages, promoting antigen presentation, and enhancing the cytotoxicity of T cells. Dysregulation of IFN-Gamma levels has been implicated in various autoimmune disorders, infectious diseases, and cancer, highlighting its importance as a biomarker for disease monitoring and therapeutic development.With its user-friendly format and accurate performance, the Human IFN-Gamma CLIA Kit from Assay Genie provides researchers with a valuable tool for investigating the role of IFN-Gamma in health and disease.
Product Name:
Human IFN-gamma (Interferon Gamma) CLIA Kit
SKU:
HUES00104
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
7.5 pg/mL
Detection range:
12.5-800 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
