The Human IFN-Gamma (Interferon-Gamma) ELISA Kit is a powerful tool for accurate detection of IFN-Gamma levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it ideal for a variety of research applications.IFN-Gamma is a key cytokine involved in the regulation of immune responses, particularly in the activation of macrophages and the enhancement of T cell function. Its dysregulation is associated with various inflammatory conditions, autoimmune diseases, and even cancer. Therefore, measuring IFN-Gamma levels can provide valuable insights into immune system function and potential disease mechanisms.With the Human IFN-Gamma ELISA Kit, researchers can quantitatively assess IFN-Gamma levels in biological samples with ease and precision, ultimately furthering our understanding of immune responses and potentially contributing to the development of new therapeutic approaches.
Product Name:
Human IFN-gamma (Interferon Gamma) ELISA Kit
SKU:
HUES01394
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
