The Human IgA (Immunoglobulin A) ELISA Kit is expertly crafted for the precise and quantitative assessment of IgA levels in a variety of human biological samples. Immunoglobulin A, a crucial antibody class, plays a vital role in the body's immune defense mechanisms, particularly in mucosal immunity, where it provides defense against pathogens trying to enter the body. Accurate measurement of IgA with our ELISA kit offers valuable insights into the immune response, antibody production, and mucosal immunity functions. Upheld by exceptional sensitivity and specificity, our ELISA kit guarantees reliable and reproducible results. Manufactured under rigorous quality control standards, this kit delivers robust, high-performance outcomes and is user-friendly for both research and clinical applications. For precise and dependable quantification of this essential antibody, trust in our Human IgA ELISA Kit.
Product Name:
Human IgA (Immunoglobulin A) ELISA Kit
SKU:
AEES00107
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
