Western blot analysis of various lysates, using Human IgA1 Rabbit mAb antibody (CAB23542) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Human IgA1 Monoclonal Antibody (CAB23542) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody specifically targets human samples and is validated for a variety of applications, including ELISA, immunofluorescence, and flow cytometry.IgA1 is the most abundant IgA subclass in the human body and plays a crucial role in defending against pathogens at mucosal surfaces. Dysfunction in IgA1 production or function has been linked to various diseases, including autoimmune disorders, gastrointestinal diseases, and infections.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Human IgA1 Monoclonal Antibody
SKU:
CAB23542
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC60394
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IgA1
Positive Sample:
Human plasma
Cellular Localization:
Secreted, Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
37KDa/42KDa
Observed MW:
60kDa
Purification Method
Affinity purification
Gene ID
3493
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Human IgA1 Rabbit mAb (CAB23542) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
