The Human IgE (Immunoglobulin E) ELISA Kit is specifically designed for the precise quantitative detection of IgE levels in various human biological samples. IgE is a key immunoglobulin involved in allergic responses, hypersensitivity reactions, and immune defense against parasites. Accurate measurement of IgE levels is crucial for studying allergic diseases, asthma, and other immunological disorders where IgE plays a significant role in disease pathology. Our IgE ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for your research needs. Manufactured under stringent quality control standards, this kit delivers robust performance and ease of use, making it an excellent choice for research applications. Trust Assay Genie's IgE ELISA Kit for dependable quantification of this important immunoglobulin in your studies.
Product Name:
Human IgE (Immunoglobulin E) ELISA Kit
SKU:
AEES00138
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
