Human IGFBP-5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit
The Human IGFBP-5 (Insulin-like Growth Factor Binding Protein 5) ELISA Kit is specifically designed for the precise measurement of IGFBP-5 levels in human biological samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results that are ideal for a variety of research applications.IGFBP-5 is a key protein involved in regulating the activity of insulin-like growth factors, playing a crucial role in cell growth, differentiation, and survival. Abnormal levels of IGFBP-5 have been associated with various diseases such as cancer, diabetes, and osteoporosis, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.For researchers seeking reliable and high-quality tools to investigate the role of IGFBP-5 in human health and disease, the Human IGFBP-5 ELISA Kit from Assay Genie is an essential addition to the laboratory toolkit.
Product Name:
Human IGFBP-5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit
SKU:
HUES01655
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
