Human IGFBP-4 (Insulin Like Growth Factor Binding Protein 4) ELISA Kit
The Human IGFBP-4 (Insulin-Like Growth Factor Binding Protein 4) ELISA Kit is specifically designed for the accurate measurement of IGFBP-4 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for various research applications.IGFBP-4 is a crucial protein that binds to insulin-like growth factors, regulating their activity and availability. It plays a significant role in cell growth, differentiation, and apoptosis, making it a valuable biomarker for studying various physiological and pathological processes.With its reliable performance and user-friendly design, the Human IGFBP-4 ELISA Kit is an essential tool for researchers interested in investigating the role of IGFBP-4 in health and disease. Don't miss out on this valuable resource for your research needs.
Product Name:
Human IGFBP-4 (Insulin Like Growth Factor Binding Protein 4) ELISA Kit
SKU:
HUES01656
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
