The Human IL-13 CLIA Kit from AssayGenie is specifically designed for the quantification of Interleukin-13 (IL-13) levels in human samples such as serum, plasma, and cell culture supernatants. IL-13 is a key cytokine involved in inflammatory and immune responses, making it a valuable biomarker for studying various diseases such as asthma, allergy, and autoimmune disorders. This CLIA kit offers high sensitivity and specificity, ensuring accurate and reliable results for your research needs. By measuring IL-13 levels, researchers can gain insights into the underlying mechanisms of these diseases and potentially identify new therapeutic targets. Trust AssayGenie's Human IL-13 CLIA Kit for your cytokine detection needs.
Product Name:
Human IL-13 CLIA Kit
SKU:
HUES00100
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
