The Rat IL-13 CLIA Kit is a reliable and accurate kit designed for the detection of IL-13 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.IL-13 is a key cytokine involved in regulating immune responses and inflammation. It plays a crucial role in allergic reactions, asthma, and other inflammatory diseases.
The ability to measure IL-13 levels accurately is essential for studying these conditions and potential therapeutic interventions.With this Rat IL-13 CLIA Kit, researchers can confidently analyze IL-13 levels in rat samples, gaining valuable insights into the role of this cytokine in various physiological and pathological processes.
Product Name:
Rat IL-13 CLIA Kit
SKU:
RTES00341
Target:
Rat IL-13
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-13. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-13, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-13. You can calculate the concentration of Rat IL-13 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-112
87-103
97-109
Average (%)
102
94
103
1:4
Range (%)
86-98
97-111
95-107
Average (%)
93
102
101
1:8
Range (%)
91-104
94-109
96-111
Average (%)
97
100
102
1:16
Range (%)
93-107
97-111
89-102
Average (%)
100
105
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
97-113
105
Cell culture media (n=5)
91-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.68
70.56
213.15
24.14
74.93
214.33
Standard deviation
3.17
7.13
20.33
2.23
8.54
24.48
C V (%)
12.34
10.1
9.54
9.24
11.4
11.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-13 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-13 in samples. No significant cross-reactivity or interference between Rat IL-13 and analogues was observed.
