The Rat IL-2 CLIA Kit is a powerful tool for measuring interleukin-2 levels in rat samples. This kit offers high sensitivity and specificity, providing accurate and reproducible results for researchers studying immune responses in rats.Interleukin-2 (IL-2) is a key cytokine involved in regulating immune responses, including T-cell activation and proliferation. Monitoring IL-2 levels can offer insights into the immune system's functioning and the development of potential treatments for autoimmune diseases, infections, and other conditions.
With the Rat IL-2 CLIA Kit, researchers can confidently analyze IL-2 levels in serum, plasma, and other sample types, advancing their understanding of immune function in rat models.
Product Name:
Rat IL-2 CLIA Kit
SKU:
RTES00013
Target:
Rat IL-2
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-2. You can calculate the concentration of Rat IL-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-117
87-98
93-109
Average (%)
108
92
100
1:4
Range (%)
97-108
96-112
94-107
Average (%)
103
102
101
1:8
Range (%)
86-97
94-106
94-112
Average (%)
91
101
102
1:16
Range (%)
99-114
94-105
98-114
Average (%)
105
100
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
102
EDTA plasma (n=5)
94-105
100
Cell culture media (n=5)
100-118
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.52
76.42
280.93
39.27
82.23
276.54
Standard deviation
4.73
9.01
18.37
3.66
7.57
18.03
C V (%)
11.12
11.79
6.54
9.32
9.21
6.52
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-2 in samples. No significant cross-reactivity or interference between Rat IL-2 and analogues was observed.
