The Rat IL-10 CLIA Kit is specifically designed for the precise quantification of IL-10 levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it a valuable tool for various research applications.IL-10, also known as interleukin-10, is a key regulatory cytokine that plays a crucial role in modulating immune responses and inflammation. Dysregulation of IL-10 has been implicated in various diseases such as autoimmune disorders, inflammatory conditions, and infectious diseases, highlighting its importance as a biomarker for studying these pathologies and developing potential therapeutic interventions.
Overall, the Rat IL-10 CLIA Kit from Assay Genie provides researchers with a reliable and efficient solution for detecting and measuring IL-10 levels in biological samples, advancing our understanding of immune regulation and disease pathology in rat models.
Product Name:
Rat IL-10 CLIA Kit
SKU:
RTES00016
Target:
Rat IL-10
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-10. You can calculate the concentration of Rat IL-10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-116
90-102
99-112
Average (%)
110
97
105
1:4
Range (%)
91-104
101-115
93-110
Average (%)
97
109
101
1:8
Range (%)
96-112
101-116
92-102
Average (%)
102
107
97
1:16
Range (%)
94-108
84-96
90-104
Average (%)
101
90
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
104
EDTA plasma (n=5)
91-104
96
Cell culture media (n=5)
97-113
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.08
77.45
249.63
24.7
75.89
241.12
Standard deviation
3.06
6.53
16.38
3.18
8.33
19.94
C V (%)
11.3
8.43
6.56
12.87
10.98
8.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-10 in samples. No significant cross-reactivity or interference between Rat IL-10 and analogues was observed.
