The Human IL-7 ELISA Kit is specifically designed for the precise measurement of interleukin-7 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.Interleukin-7 is a vital cytokine that plays a crucial role in regulating immune responses and supporting the growth and development of immune cells. Dysregulation of IL-7 has been linked to various immune-related disorders such as autoimmune diseases, inflammatory conditions, and certain types of cancer. Therefore, the detection of IL-7 levels using this ELISA kit can be valuable in understanding the underlying mechanisms of these diseases and in the development of potential therapeutic interventions. Overall, the Human IL-7 ELISA Kit from AssayGenie provides researchers with a reliable tool for studying the role of IL-7 in health and disease, offering precise and reproducible results for accurate data interpretation and further exploration.
Product Name:
Human IL-7 ELISA Kit
SKU:
HUES01809
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
