SynonymsIGSF1,IGCD1,IGDC1,INHBP,PGSF2,p120,Immunoglobulin Superfamily Member 1,Immunoglobulin-like domain-containing protein 1,Pituitary gland-specific factor 2
ReactivityHuman
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
SKU:
HUDL01538
UPC:
Product Type:
ELISA Kit
Sensitivity:
0.39ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
IGSF1,IGCD1,IGDC1,INHBP,PGSF2,p120,Immunoglobulin Superfamily Member 1,Immunoglobulin-like domain-containing protein 1,Pituitary gland-specific factor 2
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
The Human Inhibin Binding Protein (INHBP) ELISA Kit offered by AssayGenie is specifically designed for the precise measurement of INHBP levels in human biological samples such as serum, plasma, and cell culture supernatants.INHBP is a vital protein that plays a crucial role in inhibin signaling pathways, which are involved in various biological processes such as reproduction and development. By accurately measuring the levels of INHBP, researchers can gain valuable insights into its function and its impact on related conditions and diseases.
With its high sensitivity and specificity, this ELISA kit ensures accurate and reliable results, making it an excellent tool for a wide range of research applications. Whether studying reproductive disorders, hormone regulation, or developing potential therapies, the Human INHBP ELISA Kit from AssayGenie provides researchers with a valuable tool to advance their studies and discoveries.
Product Name:
Human Inhibin Binding Protein (INHBP) ELISA Kit
Product Code:
HUDL01538
Size:
96 Assays
Target:
INHBP
Synonyms:
IGSF1, IGCD1, IGDC1, INHBP, PGSF2, p120, Immunoglobulin Superfamily Member 1, Immunoglobulin-like domain-containing protein 1, Pituitary gland-specific factor 2
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
