Description
Human IST1 Recombinant Protein (N-His)
Human IST1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Glu186, with an N-terminal His tag. Predicted molecular weight: 23.44 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. IST1 homolog is a ~39 kDa protein. ESCRT-III-like protein involved in cytokinesis, nuclear envelope reassembly and endosomal tubulation. For research use only.
Is required for efficient abscission during cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2.
| Product Name: | Human IST1 Recombinant Protein (N-His) |
| SKU: | GXRP04237 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Putative MAPK-activating protein PM28, IST1, IST1 homolog, KIAA0174, hIST1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Glu186 |
| Calculated MW: | 23.44 kDa |
| UniProt: | P53990 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
