The Human Kallikrein 12 (KLK12) ELISA Kit is specifically designed for the precise measurement of kallikrein 12 levels in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for a wide array of research applications.Kallikrein 12, also known as KLK12, is an important enzyme involved in various physiological processes, including cell growth, tissue repair, and inflammation. Dysregulation of KLK12 has been linked to several diseases such as cancer and inflammatory conditions, making it a valuable biomarker for studying these ailments and developing potential therapeutic interventions.
With the Human Kallikrein 12 (KLK12) ELISA Kit, researchers can confidently measure KLK12 levels in biological samples with precision and reliability, providing valuable insights into the role of this enzyme in health and disease. Order your kit today to advance your research in the field of kallikrein biology.
Product Name:
Human Kallikrein 12 (KLK12) ELISA Kit
Product Code:
HUDL01625
Size:
96 Assays
Target:
KLK12
Synonyms:
KLK-L5, KLKL5, Kallikrein-Related Peptidase 12, Kallikrein-like protein 5
Detection Method:
Competition
Range:
15.625-1,000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
