The Human Keratin 18 (KRT18) ELISA Kit is specifically designed for the precise measurement of keratin 18 levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and offers exceptional specificity, ensuring accurate and consistent results for a variety of research purposes.Keratin 18 is a key protein found in epithelial cells, playing a crucial role in maintaining cell structure and integrity. Abnormal levels of keratin 18 have been associated with various diseases, including liver diseases, inflammatory disorders, and certain types of cancer.
As such, this ELISA kit serves as a valuable tool for investigating the role of keratin 18 in disease development and progression, as well as for identifying potential therapeutic targets.With its advanced technology and user-friendly format, the Human Keratin 18 ELISA Kit from Assay Genie is an essential resource for researchers seeking to further understand the impact of keratin 18 in human health and disease.
Product Name:
Human Keratin 18 (KRT18) ELISA Kit
Product Code:
HUDL01649
Size:
96 Assays
Target:
KRT18
Synonyms:
CK18, CYK18, K18, Cytokeratin 18, Cell proliferation-inducing gene 46 protein, Keratin, type I cytoskeletal 18
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
