The Mouse Keratin 18 (KRT18) ELISA Kit is specifically designed for the quantitative detection of keratin 18 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Keratin 18 is a key structural protein found in epithelial cells and is essential for maintaining cell integrity and function. Changes in keratin 18 levels have been associated with various diseases such as liver disease, cancer, and inflammatory conditions, making it a crucial biomarker for studying these disorders and developing targeted therapies.
The Mouse Keratin 18 ELISA Kit provides researchers with a valuable tool for investigating the role of keratin 18 in disease pathogenesis and evaluating potential treatment strategies. With its easy-to-use format and robust performance, this kit is a valuable addition to any research laboratory focused on understanding the molecular mechanisms underlying disease progression.
Product Name:
Mouse Keratin 18 (KRT18) ELISA Kit
Product Code:
MODL00778
Size:
96 Assays
Target:
KRT18
Synonyms:
CK18, CYK18, K18, Cytokeratin 18, Cell proliferation-inducing gene 46 protein, Keratin, type I cytoskeletal 18
Detection Method:
Sandwich
Range:
78.125-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
