Description
Human KNL1 Recombinant Protein (N-His)
Human KNL1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser2131-Asp2337, with an N-terminal His tag. Predicted molecular weight: 26.45 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Outer kinetochore KNL1 complex subunit KNL1 (CASC5) is a ~265 kDa protein. Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions. For research use only.
Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules. The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle. Required for kinetochore binding by a distinct subset of kMAPs (kinetochore-bound microtubule-associated proteins) and motors. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore.
| Product Name: | Human KNL1 Recombinant Protein (N-His) |
| SKU: | GXRP06367 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Blinkin, CT29, Kinetochore-null protein 1, Cancer/testis antigen 29, Bub-linking kinetochore protein, Protein CASC5, Protein D40/AF15q14, ALL1-fused gene from chromosome 15q14 protein, Kinetochore scaffold 1, CASC5, KNL1, Cancer susceptibility candidate gene 5 protein, KIAA1570, AF15q14 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ser2131-Asp2337 |
| Calculated MW: | 26.45 kDa |
| UniProt: | Q8NG31 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
