Description
Human KPNA3 Recombinant Protein (N-His)
Human KPNA3 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Thr72-Gly487, with an N-terminal His tag. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Importin subunit alpha-4 is a ~57 kDa protein. Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. For research use only.
Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
| Product Name: | Human KPNA3 Recombinant Protein (N-His) |
| SKU: | GXRP13672 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Importin alpha Q2, Importin subunit alpha-4, KPNA3, Karyopherin subunit alpha-3, QIP2, Qip2, SRP1-gamma |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Thr72-Gly487 |
| UniProt: | O00505 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
