SynonymsKLF5,BTEB2,CKLF,IKLF,Intestinal Enriched Kr?ºppel-Like Factor,Colon krueppel-like factor,Basic Transcription Element Binding Protein 2,GC-box-binding protein 2
ReactivityHuman
Sample TypeTissue Homogenates And Other Biological Fluids
SKU:
HUDL01621
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q13887
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
KLF5,BTEB2,CKLF,IKLF,Intestinal Enriched Kr?ºppel-Like Factor,Colon krueppel-like factor,Basic Transcription Element Binding Protein 2,GC-box-binding protein 2
Human Kruppel Like Factor 5, Intestinal (KLF5) ELISA Kit
The Human KLF5 (Kruppel-like Factor 5) Intestinal ELISA Kit is specifically designed for the accurate detection of KLF5 levels in human intestinal tissues and cell lysates. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.KLF5 is a key transcription factor involved in regulating cell growth, differentiation, and development in the intestinal epithelium. Dysregulation of KLF5 has been linked to various intestinal diseases, including inflammatory bowel disease and colorectal cancer, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its reliable performance and ease of use, the Human KLF5 Intestinal ELISA Kit is an essential tool for researchers seeking to investigate the role of KLF5 in intestinal health and disease.
Product Name:
Human Kruppel Like Factor 5, Intestinal (KLF5) ELISA Kit
Product Code:
HUDL01621
Size:
96 Assays
Target:
KLF5
Synonyms:
KLF5, BTEB2, CKLF, IKLF, Intestinal Enriched Krüppel-Like Factor, Colon krueppel-like factor, Basic Transcription Element Binding Protein 2, GC-box-binding protein 2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
