The Human LGALS3BP (Lectin galactoside-binding soluble 3-binding protein) ELISA Kit is specially designed for accurate detection of LGALS3BP levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications.LGALS3BP is a vital protein that plays a key role in cell adhesion, migration, and invasion processes. It is closely associated with cancer progression, immune response modulation, and inflammatory pathways, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions. Overall, the Human LGALS3BP ELISA Kit offers researchers a powerful tool to investigate the role of LGALS3BP in various physiological and pathological processes, providing valuable insights into potential diagnostic and therapeutic strategies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
