Description
Human LIMK1 Recombinant Protein (N-His)
Human LIMK1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gly419-Asp647, with an N-terminal His tag. Predicted molecular weight: 28.59 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. LIM domain kinase 1 (LIMK1) is a ~72 kDa protein. Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. For research use only.
Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation.
| Product Name: | Human LIMK1 Recombinant Protein (N-His) |
| SKU: | GXRP04948 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | LIMK-1, LIMK, LIMK1, LIM domain kinase 1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gly419-Asp647 |
| Calculated MW: | 28.59 kDa |
| UniProt: | P53667 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
