Human Lysine Specific Demethylase 4A (KDM4A) ELISA Kit
The Human Lysine-Specific Demethylase 4A (KDM4A) ELISA Kit is specially designed for the precise detection of KDM4A levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.KDM4A is a critical enzyme involved in histone demethylation, playing a key role in gene regulation and cellular processes. It is implicated in various diseases including cancer, developmental disorders, and neurological conditions, making it a valuable biomarker for studying disease mechanisms and potential therapeutic targets.
With its advanced technology and reliable performance, the Human Lysine-Specific Demethylase 4A (KDM4A) ELISA Kit is a valuable tool for researchers looking to investigate the role of KDM4A in disease pathology and explore new treatment strategies.
Product Name:
Human Lysine Specific Demethylase 4A (KDM4A) ELISA Kit
Product Code:
HUDL01602
Size:
96 Assays
Target:
KDM4A
Synonyms:
JMJD2, JMJD2A, JHDM3A, JmjC domain-containing histone demethylation protein 3A, Jumonji domain-containing protein 2A
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
