Human Lysyl Oxidase Like Protein 3 (LOXL3) ELISA Kit
The Human LOXL3 (Lysyl Oxidase-Like Protein 3) ELISA Kit is a valuable tool for the accurate quantification of LOXL3 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and consistent results, making it suitable for a variety of research purposes.LOXL3 is a key enzyme involved in extracellular matrix remodeling and plays a crucial role in various physiological processes, including tissue development and wound healing. Dysregulation of LOXL3 has been implicated in conditions such as fibrosis, cancer, and cardiovascular diseases, highlighting its significance as a potential therapeutic target and diagnostic marker.
By accurately measuring LOXL3 levels, researchers can gain a deeper understanding of its biological functions and its implications in disease pathogenesis, ultimately leading to the development of novel treatment strategies. The Human LOXL3 ELISA Kit provides a reliable platform for investigating LOXL3-related pathways and advancing research in the field of molecular biology and medicine.
Product Name:
Human Lysyl Oxidase Like Protein 3 (LOXL3) ELISA Kit
Product Code:
HUDL01739
Size:
96 Assays
Target:
LOXL3
Synonyms:
LOXL, Lysyl oxidase homolog 3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
