Human MADCAM1/Mucosal addressin cell adhesion molecule 1 ELISA Kit
The Human MadCAM1 (Mucosal Addressin Cell Adhesion Molecule 1) ELISA Kit is a powerful tool for the precise measurement of MadCAM1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is designed with exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications. MadCAM1 is an essential cell adhesion molecule that plays a critical role in immune responses within the mucosal tissues of the body. Its presence is often associated with inflammatory conditions such as inflammatory bowel disease (IBD) and is a key biomarker for understanding the mechanisms underlying these diseases.
By quantifying MadCAM1 levels, researchers can gain valuable insights into the pathogenesis of IBD and potentially identify novel therapeutic targets for intervention. Overall, the Human MadCAM1 ELISA Kit offers a reliable and efficient method for studying the role of MadCAM1 in mucosal immunity and inflammatory diseases, providing valuable data for advancing research and drug development in this field.
Product Name:
Human MADCAM1/Mucosal addressin cell adhesion molecule 1 ELISA Kit
SKU:
HUFI01484
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.188 ng/mL
Range:
0.313-20 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-100%
85-102%
87-103%
EDTA Plasma (n = 5)
83-97%
83-101%
89-101%
Heparin Plasma (n = 5)
83-97%
81-100%
82-98%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
94-102
98
EDTA Plasma (n = 5)
85-100
92
Heparin Plasma (n = 5)
88-100
96
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
