Description
Human MAPKAPK2 Recombinant Protein (N-His)
Human MAPKAPK2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Glu139-Tyr367, with an N-terminal His tag. Predicted molecular weight: 28.76 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. MAP kinase-activated protein kinase 2 (MAPKAPK2) is a ~45 kDa protein. Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. For research use only.
Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1; leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities.
| Product Name: | Human MAPKAPK2 Recombinant Protein (N-His) |
| SKU: | GXRP01679 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | MAPKAPK2, MAPKAP-K2, MAPKAP kinase 2, MAPKAPK-2, MAP kinase-activated protein kinase 2, MK2, MK-2, MAPK-activated protein kinase 2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Glu139-Tyr367 |
| Calculated MW: | 28.76 kDa |
| UniProt: | P49137 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
