Human Matrix Metalloproteinase 28 (MMP28) ELISA Kit
The Human Matrix Metalloproteinase-28 (MMP28) ELISA Kit is a specialized tool designed for the precise measurement of MMP28 levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it an invaluable asset for a variety of research endeavors.MMP28 is a key enzyme involved in extracellular matrix remodeling, playing a crucial role in tissue remodeling and wound healing processes. Dysregulation of MMP28 has been linked to various pathological conditions, including cancer metastasis, arthritis, and cardiovascular diseases, emphasizing its importance as a potential diagnostic and therapeutic target.
By utilizing the Human MMP28 ELISA Kit, researchers can gain valuable insights into the role of MMP28 in disease progression, facilitating the development of novel treatment strategies and biomarkers for improved clinical outcomes. Trust in this advanced kit to unlock new possibilities in MMP28 research and drive meaningful advancements in the field of molecular biology.
Product Name:
Human Matrix Metalloproteinase 28 (MMP28) ELISA Kit
Product Code:
HUDL01882
Size:
96 Assays
Target:
MMP28
Synonyms:
MM28, MMP25, Epilysin
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
