Description
Human MDM2 Recombinant Protein (N-His)
Human MDM2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asn3-Pro491, with an N-terminal His tag. Predicted molecular weight: 57.29 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. E3 ubiquitin-protein ligase Mdm2 is a ~55 kDa protein. E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. For research use only.
Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein.
| Product Name: | Human MDM2 Recombinant Protein (N-His) |
| SKU: | GXRP01989 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | MDM2, Hdm2, p53-binding protein Mdm2, E3 ubiquitin-protein ligase Mdm2, RING-type E3 ubiquitin transferase Mdm2, Double minute 2 protein, Oncoprotein Mdm2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asn3-Pro491 |
| Calculated MW: | 57.29 kDa |
| UniProt: | Q00987 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
