Description
Human METTL5 Recombinant Protein (N-His)
Human METTL5 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Phe209, with an N-terminal His tag. Predicted molecular weight: 25.88 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. rRNA N(6)-adenosine-methyltransferase METTL5 is a ~23 kDa protein. Catalytic subunit of a heterodimer with TRMT112, which specifically methylates the 6th position of adenine in position 1832 of 18S rRNA. For research use only.
N6-methylation of adenine(1832) in 18S rRNA resides in the decoding center of 18S rRNA and is required for translation and embryonic stem cells (ESCs) pluripotency and differentiation. 1. van Tran, N. et al. (2019) Nucleic acids research 47, 7719-7733. PMID: 31328227 2. Ignatova, VV. et al. (2020) Genes & development 34, 715-729. PMID: 32217665 3. Rong, B. et al. (2020) Cell reports 33, 108544. PMID: 33357433 4. Yu, D. et al. (2021) The Journal of biological chemistry 296, 100270. PMID: 33428944 5. Sepich-Poore, C. et al. (2022) The Journal of biological chemistry 298, 101590. PMID: 35033535
| Product Name: | Human METTL5 Recombinant Protein (N-His) |
| SKU: | GXRP07986 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Methyltransferase-like protein 5, METTL5, rRNA N6-adenosine-methyltransferase METTL5 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Phe209 |
| Calculated MW: | 25.88 kDa |
| UniProt: | Q9NRN9 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
