Description
Human MLH1 Recombinant Protein (N-His)
Human MLH1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gln407-Cys756, with an N-terminal His tag. Predicted molecular weight: 42.17 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. DNA mismatch repair protein Mlh1 is a ~84 kDa protein. Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). For research use only.
DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected.
| Product Name: | Human MLH1 Recombinant Protein (N-His) |
| SKU: | GXRP01555 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | COCA2, DNA mismatch repair protein Mlh1, MutL protein homolog 1, MLH1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gln407-Cys756 |
| Calculated MW: | 42.17 kDa |
| UniProt: | P40692 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
