Description
Human MSH3 Recombinant Protein (N-His)
Human MSH3 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Thr230-Lys526, with an N-terminal His tag. Predicted molecular weight: 35.84 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. DNA mismatch repair protein Msh3 is a ~127 kDa protein. Component of the post-replicative DNA mismatch repair system (MMR). For research use only.
Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
| Product Name: | Human MSH3 Recombinant Protein (N-His) |
| SKU: | GXRP09121 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | DNA mismatch repair protein Msh3; hMSH3; Divergent upstream protein; DUP; Mismatch repair protein 1; MRP1; MSH3; DUC1; DUG |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Thr230-Lys526 |
| Calculated MW: | 35.84 kDa |
| UniProt: | P20585 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
