Human Natriuretic Peptide Receptor 1 (NPR1) ELISA Kit
The Human Natriuretic Peptide Receptor 1 (NPR1) ELISA Kit is specifically designed for the precise measurement of NPR1 levels in human samples such as serum, plasma, and cell culture supernatants. With outstanding sensitivity and specificity, this kit delivers accurate and reproducible results, making it an indispensable tool for various research applications.NPR1 is a key receptor involved in regulating natriuretic peptides, which play a crucial role in cardiovascular homeostasis and fluid balance.
Dysregulation of NPR1 has been associated with various cardiovascular conditions, including heart failure and hypertension. As such, NPR1 serves as a valuable biomarker for investigating these diseases and developing potential therapeutic interventions.Overall, the Human NPR1 ELISA Kit from Assay Genie provides researchers with a reliable and efficient solution for studying the role of NPR1 in cardiovascular physiology and pathology.
Product Name:
Human Natriuretic Peptide Receptor 1 (NPR1) ELISA Kit
Product Code:
HUDL02071
Size:
96 Assays
Target:
NPR1
Synonyms:
ANPRA, ANPa, GUC2A, GUCY2A, NPR-A, Guanylate Cyclase A, Atrionatriuretic Peptide Receptor A, Atrial natriuretic peptide receptor type A
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
